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EMBL Course: Next Generation Sequencing: RNA Sequencing Library Preparation

EMBL Course

The goal of this course is to teach the fundamentals of NGS library preparation and validation using enrichment based tests like the Nextera / TruSight Rapid Capture assay. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and quantify the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of Enrichment based sequencing results using standard Illumina software tools like MiSeq Reporter and VariantStudio.

When

23 Apr 2018 @ 12:00 am

27 Apr 2018 @ 12:01 am

Duration: 4 days


Where

EMBL ATC

EMBL ATC

69117 Heidelberg

Germany


Organised by

European Molecular Biology Laboratory

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