Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of the feed additive containing Enterococcus faecium DSM 21913, Bifidobacterium animalis ssp. animalis DSM 16284 and Ligilactobacillus salivarius (formerly Lactobacillus salivarius) DSM 16351 (Biomin® C3) as a zootechnical feed additive (functional group: gut flora stabiliser) in the context of the renewal of the authorisation for chickens for fattening, chickens reared for laying and minor poultry species other than laying. In addition, the applicant requested the extension of use in all poultry species for fattening and reared for laying/breeding. The applicant provided evidence that the additive currently in the market complies with the conditions of the authorisation. There is no new evidence that would lead the Panel to reconsider previous conclusions that the additive is safe for the target species, consumers and the environment under the authorised conditions of use. This conclusion also applies to the target species for which a request for an extension of use is made. The Panel concluded that Biomin® C3 is not irritant to skin or eyes but should be considered as a respiratory sensitiser due to its proteinaceous nature. No conclusions could be drawn on the skin sensitisation potential of the additive. The evaluation of the efficacy is not needed in those species for which an authorisation exists. The Panel considered that the additive is efficacious in all growing poultry species at the same inclusion level of 1 × 108 CFU/kg feed and the equivalent level in water of 5 × 107 CFU/L.